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-021827 human mirna microarray v3  (Agilent technologies)


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    Structured Review

    Agilent technologies -021827 human mirna microarray v3
    021827 Human Mirna Microarray V3, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray+v3/pmc10761845-149-9-11
    Average 90 stars, based on 1 article reviews
    -021827 human mirna microarray v3 - by Bioz Stars, 2026-09
    90/100 stars

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    Generated:

    Article Title: MiR-27a as a predictor for the activation of hepatic stellate cells and hepatitis B virus-induced liver cirrhosis
    Article Snippet: RNA in the serum and cells was isolated using a mirVana PARIS kit (Ambion, Austin, TX, USA) according to the manufacturer’s protocol, which was followed by treatment with RNase-free DNase I (Promega, Madison, WI, USA) to eliminate DNA contamination. .. The profiles of serum miRNAs from 10 HBC and 10 CHB cases were generated using an Agilent Human miRNA microarray V3 (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Progression from Excessive to Deficient Syndromes in Chronic Hepatitis B: A Dynamical Network Analysis of miRNA Array Data
    Article Snippet: .. The profiles of serum miRNAs of 9 CHB patients and 10 controls were generated using Agilent Human miRNA microarray V3 (Agilent Technologies Inc., Santa Clara, CA, USA), 60 ng of RNA was labeled and hybridized for each array. .. Hybridization signals were detected with the Agilent microarray scanner; the data were extracted using Feature Extraction V10.7 (Agilent Technologies, CA, USA).

    Microarray:

    Article Title: MiR-27a as a predictor for the activation of hepatic stellate cells and hepatitis B virus-induced liver cirrhosis
    Article Snippet: RNA in the serum and cells was isolated using a mirVana PARIS kit (Ambion, Austin, TX, USA) according to the manufacturer’s protocol, which was followed by treatment with RNase-free DNase I (Promega, Madison, WI, USA) to eliminate DNA contamination. .. The profiles of serum miRNAs from 10 HBC and 10 CHB cases were generated using an Agilent Human miRNA microarray V3 (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Progression from Excessive to Deficient Syndromes in Chronic Hepatitis B: A Dynamical Network Analysis of miRNA Array Data
    Article Snippet: .. The profiles of serum miRNAs of 9 CHB patients and 10 controls were generated using Agilent Human miRNA microarray V3 (Agilent Technologies Inc., Santa Clara, CA, USA), 60 ng of RNA was labeled and hybridized for each array. .. Hybridization signals were detected with the Agilent microarray scanner; the data were extracted using Feature Extraction V10.7 (Agilent Technologies, CA, USA).

    Article Title: Comparison of short-term efficacy analysis of medium-rectal cancer surgery with robotic natural orifice specimen extraction and robotic transabdominal specimen extraction.
    Article Snippet: .. Briefly, 100 ng of total RNA from fresh-frozen tissues were labeled using miRNA Labeling Reagent (Agilent Technologies, Santa Clara, CA, USA), after which the labeled RNA was hybridized to a Human miRNA Microarray V3 (Rel 12.0, G4470C; Agilent Technologies). ..

    Article Title: Regulation of Neutrophil Senescence by MicroRNAs
    Article Snippet: .. Total RNA from highly purified neutrophils was prepared and subjected to microarray analysis using the Agilent human miRNA microarray V3. ..

    Article Title: Effects of 1α,25 dihydroxyvitamin D3 and testosterone on miRNA and mRNA expression in LNCaP cells
    Article Snippet: .. To examine the effect of T and 1,25(OH) D 3 on miRNA expression, total RNA prepared from LNCaP cells 48 h after treatment using the same experimental paradigm outlined above was analyzed on the Agilent Human miRNA microarray v3, which contains 866 human miRNAs from the Sanger database v12.0. ..

    Article Title: miR-125b and miR-100 Are Predictive Biomarkers of Response to Induction Chemotherapy in Osteosarcoma
    Article Snippet: Total RNA was then extracted using miRNeasy Mini Kit (Qiagen, Venlo, Netherlands). .. Formalin-fixed paraffin-embedded samples were sectioned at 10 μ m, and total RNA was extracted from several of such slices using the miRNeasy FFPE Kit (Qiagen). miRNA expression profiles of frozen samples were obtained by hybridizing total RNA to the Agilent human miRNA Microarray V3 (021827, 8 × 15 K, v12.0, Agilent Technologies, Santa Clara, CA), following the manufacturer's instructions. .. Hybridized microarrays were scanned with a DNA microarray scanner (Agilent G2565BA) with default protocols and settings. miRNA expression was analyzed in GeneSpring GX software (Agilent Technologies). miRNAs were considered differentially expressed when expression increased or decreased > 1.5-fold, with p < 0.05 in an unpaired t -test. mRNA expression profiles of osteosarcoma cells were obtained by hybridizing total RNA to SurePrint G3 Human GE microarray (8 × 60 K, Ver3.0, Agilent Technologies, Santa Clara, CA), following the manufacturer's instructions.

    Article Title: MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma
    Article Snippet: .. First, global microRNA profiles from 43 PA and 5 non-neoplastic brain control frozen samples were obtained with the Agilent Human miRNA Microarray V3 platform in a previously published study . .. Fold change was calculated as previously reported , comparing expression in tumor and non-neoplastic brain, after miRNA signal intensities from GeneView files were subjected to quantile normalization using GeneSpring GX, version 11, according to the standard software recommendations (Agilent Technologies).

    Article Title: MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma
    Article Snippet: .. First, we looked at a dataset of 43 PA and 5 non-neoplastic brain samples profiled with the Human miRNA Microarray V3 Agilent platform using frozen surgical tissues which tested for miR-125a-3p, miR-125a-5p, miR-125b, miR-125b-1 *and miR-125b-2*. ..

    Labeling:

    Article Title: Progression from Excessive to Deficient Syndromes in Chronic Hepatitis B: A Dynamical Network Analysis of miRNA Array Data
    Article Snippet: .. The profiles of serum miRNAs of 9 CHB patients and 10 controls were generated using Agilent Human miRNA microarray V3 (Agilent Technologies Inc., Santa Clara, CA, USA), 60 ng of RNA was labeled and hybridized for each array. .. Hybridization signals were detected with the Agilent microarray scanner; the data were extracted using Feature Extraction V10.7 (Agilent Technologies, CA, USA).

    Article Title: Comparison of short-term efficacy analysis of medium-rectal cancer surgery with robotic natural orifice specimen extraction and robotic transabdominal specimen extraction.
    Article Snippet: .. Briefly, 100 ng of total RNA from fresh-frozen tissues were labeled using miRNA Labeling Reagent (Agilent Technologies, Santa Clara, CA, USA), after which the labeled RNA was hybridized to a Human miRNA Microarray V3 (Rel 12.0, G4470C; Agilent Technologies). ..

    Purification:

    Article Title: Regulation of Neutrophil Senescence by MicroRNAs
    Article Snippet: .. Total RNA from highly purified neutrophils was prepared and subjected to microarray analysis using the Agilent human miRNA microarray V3. ..

    Expressing:

    Article Title: Effects of 1α,25 dihydroxyvitamin D3 and testosterone on miRNA and mRNA expression in LNCaP cells
    Article Snippet: .. To examine the effect of T and 1,25(OH) D 3 on miRNA expression, total RNA prepared from LNCaP cells 48 h after treatment using the same experimental paradigm outlined above was analyzed on the Agilent Human miRNA microarray v3, which contains 866 human miRNAs from the Sanger database v12.0. ..

    Article Title: miR-125b and miR-100 Are Predictive Biomarkers of Response to Induction Chemotherapy in Osteosarcoma
    Article Snippet: Total RNA was then extracted using miRNeasy Mini Kit (Qiagen, Venlo, Netherlands). .. Formalin-fixed paraffin-embedded samples were sectioned at 10 μ m, and total RNA was extracted from several of such slices using the miRNeasy FFPE Kit (Qiagen). miRNA expression profiles of frozen samples were obtained by hybridizing total RNA to the Agilent human miRNA Microarray V3 (021827, 8 × 15 K, v12.0, Agilent Technologies, Santa Clara, CA), following the manufacturer's instructions. .. Hybridized microarrays were scanned with a DNA microarray scanner (Agilent G2565BA) with default protocols and settings. miRNA expression was analyzed in GeneSpring GX software (Agilent Technologies). miRNAs were considered differentially expressed when expression increased or decreased > 1.5-fold, with p < 0.05 in an unpaired t -test. mRNA expression profiles of osteosarcoma cells were obtained by hybridizing total RNA to SurePrint G3 Human GE microarray (8 × 60 K, Ver3.0, Agilent Technologies, Santa Clara, CA), following the manufacturer's instructions.

    Formalin-fixed Paraffin-Embedded:

    Article Title: miR-125b and miR-100 Are Predictive Biomarkers of Response to Induction Chemotherapy in Osteosarcoma
    Article Snippet: Total RNA was then extracted using miRNeasy Mini Kit (Qiagen, Venlo, Netherlands). .. Formalin-fixed paraffin-embedded samples were sectioned at 10 μ m, and total RNA was extracted from several of such slices using the miRNeasy FFPE Kit (Qiagen). miRNA expression profiles of frozen samples were obtained by hybridizing total RNA to the Agilent human miRNA Microarray V3 (021827, 8 × 15 K, v12.0, Agilent Technologies, Santa Clara, CA), following the manufacturer's instructions. .. Hybridized microarrays were scanned with a DNA microarray scanner (Agilent G2565BA) with default protocols and settings. miRNA expression was analyzed in GeneSpring GX software (Agilent Technologies). miRNAs were considered differentially expressed when expression increased or decreased > 1.5-fold, with p < 0.05 in an unpaired t -test. mRNA expression profiles of osteosarcoma cells were obtained by hybridizing total RNA to SurePrint G3 Human GE microarray (8 × 60 K, Ver3.0, Agilent Technologies, Santa Clara, CA), following the manufacturer's instructions.

    Control:

    Article Title: MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma
    Article Snippet: .. First, global microRNA profiles from 43 PA and 5 non-neoplastic brain control frozen samples were obtained with the Agilent Human miRNA Microarray V3 platform in a previously published study . .. Fold change was calculated as previously reported , comparing expression in tumor and non-neoplastic brain, after miRNA signal intensities from GeneView files were subjected to quantile normalization using GeneSpring GX, version 11, according to the standard software recommendations (Agilent Technologies).



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    Image Search Results


    A miRNA microarray cluster was used to demonstrate the ability of HTLV-1 Tax to globally downregulate the expression of cellular miRNAs. The two lanes represent the Cy5/Cy3 ratio in dye flip. The miRNAs isolated from the stably integrated pooled Jurkat clone transfected with Tax were labeled with Cy5 whereas those isolated from the same integrated clone but transfected with control plasmid (pCMV4) were labeled with Cy3. The table shows the high probability miRNAs against P/CAF and p300 that have been downregulated in the presence of HTLV-1 Tax.

    Journal: PLoS ONE

    Article Title: HTLV-1 Tax Mediated Downregulation of miRNAs Associated with Chromatin Remodeling Factors in T Cells with Stably Integrated Viral Promoter

    doi: 10.1371/journal.pone.0034490

    Figure Lengend Snippet: A miRNA microarray cluster was used to demonstrate the ability of HTLV-1 Tax to globally downregulate the expression of cellular miRNAs. The two lanes represent the Cy5/Cy3 ratio in dye flip. The miRNAs isolated from the stably integrated pooled Jurkat clone transfected with Tax were labeled with Cy5 whereas those isolated from the same integrated clone but transfected with control plasmid (pCMV4) were labeled with Cy3. The table shows the high probability miRNAs against P/CAF and p300 that have been downregulated in the presence of HTLV-1 Tax.

    Article Snippet: The purified tagged miRNA was hybridized to an NCode Human miRNA Microarray V3 (Invitrogen), which is an epoxy-coated glass slide printed with miRNA probes targeting all of the known human miRNAs in the miRBase Sequence database, release 10.0, in an antisense orientation.

    Techniques: Microarray, Expressing, Isolation, Stable Transfection, Transfection, Labeling, Plasmid Preparation